mouse anti myd88  (Proteintech)

Journal: Biomarker Research

Article Title: S100A9 as a potential novel target for experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome

doi: 10.1186/s40364-025-00763-5

Figure Lengend Snippet: Knockdown of S100A9 significantly reduced TLR4/NF-κB and TLR4/p38 signalling pathway activating and decreasing inflammation and apoptosis-related protein expression in EAC mice ( n = 6). GSEA analysis showing that TLR4/MyD88, NF-κB, and p38 signalling are activated in IC/BPS patients and EAC mice. ( B - C ) Knockdown of S100A9 significantly reduced TLR4/NF-κB and TLR4/p38 signalling pathway protein expression in EAC mice. ( D - E ) Knockdown of S100A9 significantly reduced the expression of bladder inflammation-related proteins (IL-6, IL-1β and TNF-α) in EAC mice. ( F ) GSEA analysis showing that apoptosis signalling is activated in IC/BPS patients and EAC mice. ( G - H ) Knockdown of S100A9 expression significantly reduced the expression of bladder apoptosis-related proteins (Bax, caspase-3, caspase-8, and caspase-1) and improved the expression of epithelial damage marker proteins (UPK3A and UPK2) in EAC mice. NS indicates no difference; * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The antibodies used in this study were as follows: rabbit monoclonal anti-S100A9 antibody (1:800, 73425, CST, USA), mouse monoclonal anti-TLR4 antibody (1:1000, MA5-16216, Thermo Fisher, USA), mouse monoclonal anti-MyD88 antibody (1:1000, 67969-1-Ig, Proteintech, China), rabbit polyclonal anti-P38 (1:1000, B-IO-10130, Biozellen, USA), rabbit polyclonal p-p38 (1:800, B-IO-10131, Biozellen, USA), rabbit monoclonal anti-NF-κB (1:1000, 8242, CST, USA), rabbit monoclonal anti-p-NF-κB (1:800, 3033, CST, USA), mouse monoclonal anti-IκBα antibody (1:1000, 4814, CST, USA), mouse monoclonal anti-IκBα antibody (1:1000, 4814, CST, USA), rabbit monoclonal anti-IκBα antibody (1:1000, 2859, CST, USA), mouse monoclonal anti-IL-1β (1:800, sc-12742, Santa Cruz Biotechnology, USA), mouse monoclonal anti-IL-6 (1:500, sc-57315, Santa Cruz Biotechnology, USA), TNF-α (1:1000, 17590-1-AP, Proteintech, China), rabbit polyclonal anti-TNF-α (1:600, 17590-1-AP, Proteintech, China), mouse monoclonal anti-GAPDH (1:1000, 60004-1-Ig, Proteintech, China), mouse monoclonal anti-caspase-3 (1:600, 66470-2-Ig, Proteintech, China), rabbit polyclonal anti-caspase-8 (1:600, 13423-1-AP, Proteintech, China), rabbit monoclonal anti-caspase-1 antibody (1:800, 24232, CST, USA), mouse monoclonal anti-Bax (1:1000, 60267-1-Ig, Proteintech, China), mouse monoclonal anti-UPK3A (1:800, sc-166808, Santa Cruz Biotechnology, USA), rabbit polyclonal anti-UPK2 (1:600, 21149-1-AP, Proteintech, China), goat anti-rabbit secondary antibody (1:2000, G6120, Thermo Fisher, USA) and goat anti-mouse secondary antibody (1:2000, G21040 , Thermo Fisher, USA).

Techniques: Knockdown, Expressing, Marker